4-Hydroxy Tolbutamide is primarily used in the field of medical research and pharmacology. It serves as a metabolite of Tolbutamide, a first-generation sulfonylurea drug used to manage type 2 diabetes. Researchers study 4-Hydroxy Tolbutamide to understand the metabolic pathways and pharmacokinetics of Tolbutamide, which helps in assessing drug efficacy and safety. This compound is also utilized in drug metabolism studies to evaluate how the body processes and eliminates Tolbutamide, providing insights into potential drug interactions and side effects. Additionally, it aids in the development of analytical methods for detecting and quantifying Tolbutamide and its metabolites in biological samples, contributing to improved therapeutic monitoring and personalized medicine approaches.