1'-Hydroxy Bufuralol is primarily used in pharmacological research as a metabolite of bufuralol, a beta-adrenergic blocking agent. It plays a significant role in studying the metabolic pathways and pharmacokinetics of bufuralol, helping researchers understand how the body processes and eliminates the drug. This compound is particularly valuable in investigating the cytochrome P450 enzyme system, specifically CYP2D6, which is responsible for its formation. By studying 1'-Hydroxy Bufuralol, scientists can gain insights into interindividual variability in drug metabolism, which is crucial for personalized medicine and optimizing drug efficacy and safety. Additionally, it is used in in vitro and in vivo studies to assess drug-drug interactions and the impact of genetic polymorphisms on metabolic activity.