Used primarily as a photoaffinity labeling agent in biochemical and pharmacological research. Its diazirine group generates highly reactive carbenes upon exposure to UV light, enabling covalent binding to nearby biomolecules such as proteins or nucleic acids. This property makes it valuable for studying molecular interactions, mapping binding sites, and identifying unknown targets of bioactive compounds. The iodophenyl moiety allows for easy incorporation of radioactive or fluorescent tags, facilitating detection and quantification. Commonly employed in probe synthesis for target identification and validation in drug discovery.