Monodispersed magnetite microspheres are widely used in biomedical applications due to their magnetic properties and uniform size. They serve as effective carriers in drug delivery systems, enabling targeted treatment of diseases such as cancer by guiding the microspheres to specific sites using external magnetic fields. In diagnostics, these microspheres are employed in magnetic resonance imaging (MRI) as contrast agents to enhance image clarity. They are also utilized in magnetic separation techniques, where they help isolate specific biomolecules, cells, or pathogens from complex mixtures, streamlining processes in research and clinical laboratories. Additionally, their use in hyperthermia therapy for cancer treatment involves generating heat under an alternating magnetic field to destroy tumor cells. Their uniformity and magnetic responsiveness make them valuable in various advanced technological and medical applications.