Monodispersed magnetite microspheres are widely used in biomedical applications due to their magnetic properties and uniform size. They serve as effective carriers for targeted drug delivery, enabling precise treatment of diseases such as cancer by concentrating therapeutic agents at specific sites. In diagnostics, these microspheres are utilized in magnetic resonance imaging (MRI) as contrast agents to enhance image clarity. They also play a crucial role in bioseparation processes, where they are employed to isolate and purify biomolecules like DNA, proteins, and cells from complex mixtures. Additionally, their use in hyperthermia treatment for cancer involves generating heat under an alternating magnetic field to destroy tumor cells. In environmental applications, they are applied for wastewater treatment to remove heavy metals and other contaminants efficiently. Their versatility and functionality make them valuable in both scientific research and industrial applications.