Used in lipid nanoparticle (LNP) formulations for targeted drug and gene delivery, especially in mRNA vaccines and therapies. Its positively charged head group enhances nucleic acid binding and cellular uptake. The unsaturated oleoyl chains provide membrane fluidity, improving fusion with cell membranes and endosomal escape. Commonly applied in research and development of non-viral delivery systems for cancer therapy, genetic disorders, and infectious disease vaccines. Stable under controlled conditions and compatible with other ionizable lipids, cholesterol, and PEGylated lipids in LNP mixtures.