Used primarily in genetic research to study mutation processes, this chemical induces frameshift mutations in DNA, making it a valuable tool for understanding genetic mechanisms and DNA repair pathways. It is also employed in microbial genetics to analyze the effects of mutations on bacterial genomes. Additionally, it serves as a reference mutagen in assays to evaluate the mutagenic potential of other substances, contributing to safety assessments in environmental and pharmaceutical studies. Its role in identifying and characterizing mutagens helps advance knowledge in genetic toxicology and cancer research.