Di(N-succinimidyl) 3,3'-Dithiodipropionate is widely used as a cross-linking reagent in biochemical and bioconjugation applications. Its primary function is to create stable covalent bonds between molecules, particularly proteins, peptides, and other biomolecules. The reagent contains a disulfide bond, which allows for reversible cross-linking under reducing conditions, making it useful for studying protein-protein interactions and molecular dynamics. It is also employed in the preparation of immunoconjugates, enzyme labeling, and the development of drug delivery systems. Additionally, its ability to form cleavable linkages makes it valuable in research involving the isolation and identification of protein complexes.