Used primarily in bioconjugation and click chemistry applications, this compound facilitates the linking of biomolecules due to its dual functional groups. The biotin moiety allows for strong binding to avidin or streptavidin, enabling detection or purification processes in biochemical assays. The DBCO group participates in strain-promoted azide-alkyne cycloaddition (SPAAC), a type of click chemistry, which is highly efficient and selective for labeling or modifying biomolecules without the need for catalysts. The PEG3 spacer enhances solubility and reduces steric hindrance, improving the efficiency of the conjugation process. Additionally, the disulfide bond (SS) provides a cleavable linkage, allowing for controlled release or separation of conjugated molecules under reducing conditions. This makes it valuable in targeted drug delivery, diagnostic imaging, and proteomics research.