Boc-Gly-Gly-OH is widely used in peptide synthesis as a building block for creating more complex peptide structures. Its Boc (tert-butoxycarbonyl) protecting group ensures selective reactions during peptide chain elongation, preventing unwanted side reactions. This compound is particularly useful in solid-phase peptide synthesis (SPPS), where it serves as a precursor for dipeptides or longer sequences. It is also employed in research to study enzyme-substrate interactions, peptide folding, and biochemical pathways. Additionally, Boc-Gly-Gly-OH is utilized in the development of peptide-based drugs and biomaterials, contributing to advancements in medicinal chemistry and biotechnology.