Cyclo(-D-Ala-Val) is primarily utilized in peptide research and drug development due to its cyclic structure, which enhances stability and bioavailability. It serves as a building block in the synthesis of more complex peptide-based compounds, particularly in the design of antimicrobial and anticancer agents. Its unique conformation allows it to mimic natural peptide motifs, making it valuable for studying protein-protein interactions and enzyme inhibition. Additionally, it is employed in the development of peptidomimetics, which are used to create therapeutic agents with improved pharmacological properties.