DL-Alanyl-DL-valine finds its application primarily in the field of biochemical research and peptide synthesis. It is utilized as a building block in the creation of more complex peptides and proteins, aiding in the study of their structure and function. This compound is also used in the development of nutritional supplements, where it contributes to the formulation of amino acid-based products designed to support muscle recovery and overall health. Additionally, it serves as a substrate in enzymatic studies, helping researchers understand the mechanisms of enzyme action and specificity. Its role in these areas makes it a valuable tool in both scientific and industrial contexts.