This chemical is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS) methods. It serves as a protected dipeptide building block, enabling the sequential assembly of peptides with high precision. The Boc (tert-butyloxycarbonyl) group protects the amine functionality, while the Fmoc (fluorenylmethyloxycarbonyl) group provides orthogonal protection for the amine of the threonine residue. The tert-butyl (tBu) group protects the hydroxyl side chain of threonine. This compound is especially useful in synthesizing complex peptides with specific sequences, as it allows for selective deprotection and coupling steps. Its application is critical in producing peptides for research, pharmaceuticals, and biotechnology, where precise control over peptide structure is essential.