DL-Alanyl-DL-leucine finds its application primarily in the field of biochemical research and peptide synthesis. It is used as a building block in the preparation of more complex peptides and proteins, aiding in the study of their structure and function. This compound is also utilized in enzymatic studies to understand the mechanisms of peptide bond formation and hydrolysis. Additionally, it serves as a substrate for various proteases and peptidases, helping to characterize their activity and specificity. In the pharmaceutical industry, it can be employed in the development of peptide-based drugs, contributing to the design of therapeutic agents targeting specific biological pathways. Its role in nutritional science includes being a component in synthetic diets to study amino acid metabolism and requirements in organisms.