Z-ALA-VAL-OH is primarily used in peptide synthesis and biochemical research. It serves as a building block for creating more complex peptide structures, aiding in the study of protein interactions and enzyme mechanisms. This compound is also utilized in the development of peptide-based drugs, where it helps in understanding the structure-activity relationships essential for drug design. Additionally, it finds application in the field of proteomics for peptide mapping and sequence analysis, contributing to the identification and characterization of proteins. Its role in mimicking natural peptide sequences makes it valuable in studying biological processes and designing therapeutic agents.