H-Leu-Met-OH is primarily used in peptide synthesis and research applications. It serves as a building block for creating larger peptides or proteins, particularly in studies focusing on structure-activity relationships. This dipeptide is also utilized in biochemical research to investigate enzyme interactions, metabolic pathways, and peptide-based drug development. Additionally, it can be employed in the study of amino acid transport mechanisms and as a reference compound in analytical chemistry for peptide identification and characterization.