Freund’s Adjuvant is widely used in immunology research to enhance the immune response to antigens. It is particularly valuable in the production of polyclonal antibodies, where it helps stimulate a stronger and more sustained immune reaction. Complete Freund’s Adjuvant (CFA) is often used for the initial immunization due to its ability to induce a robust immune response, while Incomplete Freund’s Adjuvant (IFA) is typically used for booster injections to maintain immunity without causing excessive inflammation. This adjuvant is also employed in vaccine development to improve the efficacy of experimental vaccines by promoting a more effective antigen presentation. Additionally, it is used in studies focusing on autoimmune diseases and inflammation, as it can induce experimental models of these conditions in laboratory animals.