Used as a deuterated solvent in nuclear magnetic resonance (NMR) spectroscopy to enable precise structural analysis of organic and biomolecules without interference from proton signals. Its fully deuterated structure ensures high spectral clarity and improves measurement accuracy. Also employed as a labeled internal standard in mass spectrometry-based metabolic studies, allowing reliable tracking of biochemical pathways. Due to its stability and solubility, it serves as a heavy analog of glycerol in kinetic isotope effect research and deuteration experiments.