Used as a deuterated solvent in nuclear magnetic resonance (NMR) spectroscopy to provide a clear background signal without interfering hydrogen peaks. Its deuterium atoms allow for signal locking in NMR instruments while minimizing proton interference. Also employed as a tracer in metabolic and pharmacokinetic studies due to its stability and detectability in biological systems. Serves as a reference compound in mass spectrometry and isotopic labeling experiments. Preferred in analytical chemistry for calibrating instruments and validating methods where hydrogen-free or deuterium-enriched environments are required.