Used primarily as a deuterated reagent in nuclear magnetic resonance (NMR) spectroscopy, 2-picoline-D7 serves as a solvent or internal standard for analyzing reaction mechanisms and molecular structures. Its deuterium labeling allows for clear distinction from protonated compounds, minimizing signal interference in NMR spectra. It is also employed in kinetic isotope effect studies and in the synthesis of deuterium-labeled pharmaceuticals and agrochemicals where tracking molecular transformations is essential.