(R,S)-Anatabine-2,4,5,6-d4 is primarily used in research and analytical applications, particularly in the field of pharmacology and biochemistry. It serves as a deuterated internal standard in mass spectrometry studies, enabling accurate quantification of anatabine and its metabolites in biological samples. This compound is valuable in studying the pharmacokinetics and metabolic pathways of anatabine, a naturally occurring alkaloid. Additionally, it aids in the development of diagnostic methods and therapeutic monitoring, contributing to advancements in understanding tobacco-related compounds and their effects on human health.