Aztreonam-d6 is primarily used as an internal standard in analytical chemistry for the quantification of aztreonam in various biological samples. Its deuterated form ensures minimal interference during mass spectrometry, providing accurate and reliable measurements. This application is crucial in pharmacokinetic studies, where precise monitoring of drug concentrations in plasma, urine, or tissues is required. Additionally, it aids in the development and validation of analytical methods, ensuring consistency and reproducibility in research and clinical settings. Its use is particularly valuable in studies involving antibiotic efficacy, drug metabolism, and therapeutic drug monitoring.