Used primarily as an internal standard in mass spectrometry and gas chromatography for the accurate quantification and identification of long-chain alkanes in complex mixtures. Its deuterated structure provides a distinct mass shift, enabling clear differentiation from non-labeled analogs. Commonly applied in environmental analysis to track hydrocarbon contamination and biodegradation processes. Also utilized in metabolic studies and petroleum research to monitor the behavior and distribution of high molecular weight alkanes under various conditions.