Used as a stable isotope-labeled internal standard in mass spectrometry-based bioanalytical studies, particularly in quantitative proteomics and metabolomics. Its deuterated structure provides a distinct mass shift, enabling accurate differentiation from non-labeled analogs during detection. Commonly applied in buffer systems where precise pH control and isotopic consistency are critical, such as in enzymatic assays and protein separation techniques. Also utilized in NMR spectroscopy for signal referencing and structural analysis due to its well-defined spectral properties and chemical stability.