Used as a stable isotope-labeled internal standard in mass spectrometry and gas chromatography for the detection and quantification of ethyl acetate and related esters in environmental, biological, and food samples. Its deuterated structure allows clear differentiation from non-labeled compounds during analysis, improving accuracy in trace-level measurements. Commonly applied in metabolic studies, pharmacokinetics, and residue analysis where precise tracking of ester hydrolysis or acetic acid derivatives is required. Also employed in NMR spectroscopy as a solvent or reference compound due to the distinct signal from deuterium atoms.