L-Tyrosine-3,5-D2 is primarily used in research and scientific studies as a labeled compound. Its deuterium atoms make it a valuable tool in metabolic pathway analysis, allowing researchers to track and study the behavior of tyrosine in biological systems. It is often employed in nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry experiments to gain insights into protein synthesis, neurotransmitter production, and other biochemical processes. Additionally, it aids in the development of diagnostic methods and the study of diseases related to tyrosine metabolism, such as phenylketonuria (PKU). Its labeled structure also supports the investigation of drug metabolism and pharmacokinetics in pharmaceutical research.