DL-Aspartic-2,3,3-D3 acid is primarily used in research and analytical applications, particularly in the field of isotope labeling and metabolic studies. Its deuterium-labeled structure makes it a valuable tool for tracing and understanding biochemical pathways, especially in amino acid metabolism. Researchers utilize it in nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry to study protein interactions, enzyme mechanisms, and metabolic processes in cells. It is also employed in the development of pharmaceuticals to investigate drug metabolism and pharmacokinetics, providing insights into how compounds are processed in biological systems. Additionally, it serves as a stable isotope-labeled internal standard in quantitative analysis, ensuring accuracy in measuring aspartic acid levels in various samples.