Used in metabolic studies and research involving amino acid metabolism, particularly to track and analyze biochemical pathways. It serves as a labeled tracer in nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry, enabling detailed investigation of protein synthesis and degradation processes. Applied in pharmaceutical research to study drug interactions with amino acids and to develop targeted therapies. Also utilized in nutritional science to understand the role of alanine in dietary and physiological contexts. Its deuterium labeling allows for precise monitoring in complex biological systems.