Used in metabolic research and stable isotope tracing studies, this deuterated compound enables precise tracking of glutamine metabolism in biological systems. Its primary application lies in mass spectrometry-based assays where the deuterium label allows differentiation between endogenous and labeled metabolites. It is particularly valuable in cancer research, immunology, and neuroscience to study metabolic flux in cells and tissues. The compound also supports pharmaceutical development by helping assess drug effects on metabolic pathways involving glutamine. Due to its stability and specificity, it is widely used in tracer experiments to investigate amino acid transport, protein synthesis, and nitrogen metabolism.