Used as a stable isotope-labeled internal standard in mass spectrometry and analytical chemistry for accurate quantification of ethylene diamine and related compounds. Its deuterated structure allows clear differentiation from non-labeled analogs in biological, environmental, and pharmaceutical samples. Commonly applied in metabolic studies, reaction mechanism tracing, and quality control where precise detection and measurement are critical. Also utilized in NMR spectroscopy as a reference or probe due to the distinct signal from deuterium atoms.