IU1-47 is a selective inhibitor of USP14, a deubiquitinating enzyme associated with the proteasome. It is primarily used in research settings to study protein degradation pathways, particularly the ubiquitin-proteasome system. By inhibiting USP14, IU1-47 enhances the degradation of ubiquitinated proteins, making it a valuable tool for investigating cellular protein homeostasis. It has shown potential in neurodegenerative disease research, where accumulation of misfolded proteins is a key feature. IU1-47 has been used in cellular and animal models to explore its effects on clearing toxic protein aggregates, such as tau or TDP-43. Additionally, it is employed in cancer research due to the proteasome’s role in cell cycle regulation and apoptosis, helping to understand how modulating deubiquitination can affect tumor cell survival. Its application remains mainly in preclinical research, aiding in target validation and pathway analysis rather than clinical use.