Used as a derivatization reagent in analytical chemistry, particularly for the determination of fatty acids by gas chromatography. It efficiently converts free fatty acids and their glycerides into methyl esters, making them more volatile and suitable for separation and detection. Commonly applied in the analysis of fats, oils, and biological samples where accurate profiling of fatty acid composition is required. The reagent offers a convenient one-step reaction under mild conditions, reducing preparation time and improving reproducibility. Also used in the synthesis of methyl esters for biodiesel quality control and in environmental testing of lipid-containing samples.