Guanidine hydrochloride is widely used in molecular biology for protein denaturation. It helps in unfolding proteins by disrupting hydrogen bonds and hydrophobic interactions, making it valuable in studying protein structure and function. It is also employed in RNA extraction processes, where it aids in breaking down cell membranes and inactivating nucleases, ensuring the integrity of RNA samples. Additionally, it serves as a chaotropic agent in affinity chromatography, facilitating the purification of proteins and nucleic acids. In biochemistry, it is utilized to solubilize inclusion bodies, enabling the refolding of recombinant proteins. Its role in these applications highlights its importance in research and biotechnological processes.