Formate dehydrogenase from Candida boidinii is widely used in biochemical research and industrial applications due to its ability to catalyze the oxidation of formate to carbon dioxide while reducing NAD+ to NADH. This enzyme is particularly valuable in enzymatic assays for measuring formate concentration or monitoring NADH production in various metabolic studies. In biotechnology, it plays a critical role in cofactor regeneration systems, enabling sustainable and cost-effective enzymatic synthesis of pharmaceuticals and fine chemicals. Additionally, it is employed in biosensors for detecting formate in environmental and industrial samples, ensuring accurate monitoring of formate levels. Its stability and efficiency make it a preferred choice for applications requiring precise and reliable enzymatic activity.