Used primarily in biochemical research, this enzyme plays a critical role in the study of metabolic pathways, particularly those involving the conversion of mannitol to fructose. It is also utilized in the development of biosensors for detecting and quantifying mannitol in various samples, which is important in food quality control and medical diagnostics. Additionally, its application extends to the production of low-calorie sweeteners and in the fermentation industry for enhancing the efficiency of sugar metabolism in microbial processes.