Isocitric Dehydrogenase (NADP), sourced from porcine heart, is widely utilized in biochemical research and diagnostic applications. It plays a critical role in the citric acid cycle, where it catalyzes the oxidative decarboxylation of isocitrate to α-ketoglutarate, while reducing NADP+ to NADPH. This reaction is essential for generating NADPH, a key cofactor in biosynthetic processes and cellular antioxidant defense mechanisms. In research, this enzyme is employed to study metabolic pathways, particularly those involving energy production and redox balance. It is also used in enzymatic assays to measure isocitrate levels or to monitor NADPH production, which can indicate the activity of the citric acid cycle in various biological samples. Additionally, it serves as a tool in the development of diagnostic kits for metabolic disorders, where alterations in isocitrate or NADPH levels may indicate disease states. Its specificity and efficiency make it a valuable component in both academic and clinical settings.