Leucine dehydrogenase is widely used in biochemical research and industrial applications for its role in catalyzing the reversible deamination of L-leucine and other branched-chain amino acids. It is particularly valuable in enzymatic assays to measure the concentration of leucine or other amino acids in biological samples, aiding in metabolic studies and diagnostics. In biotechnology, it is employed in the production of chiral compounds and intermediates for pharmaceuticals due to its high specificity and efficiency. Additionally, leucine dehydrogenase is utilized in biosensors for detecting amino acids in food and environmental samples, ensuring quality control and safety. Its application in biocatalysis also supports the development of sustainable chemical processes by enabling green synthesis pathways.