Used in biochemical research to study steroid metabolism and hormone regulation. It plays a key role in converting steroid hormones, aiding in understanding endocrine disorders. Applied in diagnostic kits to measure steroid levels in clinical samples, helping diagnose conditions like adrenal insufficiency or congenital adrenal hyperplasia. Also utilized in pharmaceutical development to test drugs targeting steroid-related pathways. Its enzymatic activity is crucial for producing specific steroid derivatives used in research and therapy.