L-Alanine Dehydrogenase from Bacillus subtilis is widely used in biochemical research and industrial applications. It plays a crucial role in enzymatic assays for the quantitative determination of L-alanine and pyruvate, making it valuable in metabolic studies and diagnostics. In biotechnology, it is employed in the production of L-alanine through enzymatic conversion, which is essential in the synthesis of pharmaceuticals, food additives, and biodegradable plastics. Additionally, it is utilized in biosensors for monitoring alanine levels in biological samples, aiding in clinical diagnostics and research. Its stability and specificity make it a preferred enzyme in various biotechnological processes.