3-Hydroxybutyrate dehydrogenase is widely used in biochemical research and medical diagnostics. It plays a crucial role in the measurement of ketone bodies, particularly in the assessment of metabolic disorders such as diabetes and ketosis. The enzyme is employed in enzymatic assays to quantify levels of 3-hydroxybutyrate, a key indicator of fatty acid metabolism and energy utilization in the body. Additionally, it is utilized in studies related to mitochondrial function and energy metabolism, providing insights into cellular health and disease mechanisms. Its application extends to the development of biosensors for real-time monitoring of metabolic states in clinical and research settings.