L-Phenylalanine dehydrogenase is widely used in biochemical research and industrial applications due to its ability to catalyze the reversible deamination of L-phenylalanine to phenylpyruvate. In the pharmaceutical industry, it plays a crucial role in the synthesis of chiral compounds, particularly in the production of enantiomerically pure amino acids, which are essential for drug development. It is also employed in diagnostic kits for measuring L-phenylalanine levels in blood, aiding in the detection and management of phenylketonuria (PKU), a genetic disorder. Additionally, this enzyme is utilized in biosensors and bioassays for monitoring amino acid metabolism in various biological systems. Its specificity and efficiency make it a valuable tool in enzymatic assays and biotechnological processes.