Used in traditional medicine for its analgesic and anti-inflammatory properties, particularly in treating liver and biliary tract disorders. In pharmaceutical research, (-)-Chelidonine exhibits anti-cancer effects by inhibiting cell proliferation through disruption of mitosis, such as microtubule inhibition, and inducing apoptosis in certain tumor cells, showing potential as an anti-tumor drug. It is also studied for its antispasmodic effects on smooth muscle, relevant for gastrointestinal disorders (e.g., stomach issues or irritable bowel syndrome) and uterine spasm relief. Additionally, it is investigated for neuroprotective activity, with evidence suggesting it may influence acetylcholine levels, which is relevant for cognitive disorders. However, it remains in the research stage and requires further data to confirm safety and efficacy for human use.