N-(2-Nitrophenylsulfenyl)-L-isoleucine dicyclohexylammonium salt is a protected amino acid derivative used primarily in peptide synthesis. The 2-nitrophenylsulfenyl (Nps) group serves as a protecting group for the α-amino group of L-isoleucine, enabling selective activation and coupling to other amino acids during solid-phase or solution-phase synthesis. Deprotection is achieved under mild conditions, such as treatment with thiols or dilute acid, preserving the integrity of the growing peptide chain. This reagent is particularly useful in the synthesis of complex peptides and peptidomimetics for research in organic chemistry and medicinal chemistry, including the development of pharmaceutical intermediates and biologically active molecules.