Calpeptin is widely used in biochemical research as a potent and selective inhibitor of calpain, a family of calcium-dependent proteases. It is particularly valuable in studying the role of calpain in various cellular processes, such as apoptosis, cell signaling, and cytoskeletal remodeling. Researchers utilize Calpeptin to investigate its protective effects in neurodegenerative diseases, where calpain overactivation is implicated in neuronal damage. Additionally, it is employed in studies related to ischemia-reperfusion injury, as it helps in understanding the mechanisms of cell death and survival under stress conditions. Calpeptin is also used in cancer research to explore its potential in inhibiting tumor cell migration and invasion, offering insights into therapeutic strategies. Its application extends to cardiovascular research, where it aids in examining the role of calpain in heart diseases and muscle degeneration. Overall, Calpeptin serves as a critical tool in elucidating the physiological and pathological functions of calpain across various fields of biomedical science.