BOC-D-FMK is widely used in biochemical research as a potent and selective inhibitor of caspases, which are enzymes involved in apoptosis (programmed cell death). Its application is particularly significant in studying the mechanisms of apoptosis and identifying the roles of specific caspases in cellular processes. Researchers utilize it to inhibit caspase activity in cell cultures, allowing them to observe the effects of blocked apoptosis and understand its implications in diseases such as cancer, neurodegenerative disorders, and autoimmune conditions. Additionally, BOC-D-FMK is employed in drug discovery and development to screen for potential therapeutic agents targeting apoptosis pathways. Its ability to permeate cells makes it a valuable tool for in vitro and ex vivo experiments, providing insights into cell survival and death mechanisms.