Calpain Inhibitor VI (SJA 6017) is widely used in biomedical research to study the role of calpain proteases in various cellular processes. It is particularly valuable in investigating conditions such as neurodegenerative diseases, muscle degeneration, and ischemic injuries, where calpain activity is often dysregulated. Researchers use this inhibitor to block calpain-mediated protein cleavage, helping to understand its impact on cell death, cytoskeletal integrity, and signal transduction pathways. Additionally, it serves as a tool in drug discovery efforts aimed at developing therapeutic agents for diseases associated with excessive calpain activity. Its application extends to in vitro and in vivo models, providing insights into potential treatment strategies for conditions like Alzheimer's disease, stroke, and muscular dystrophy.