Calpain inhibitor-1 is widely used in biochemical research to study the role of calpain enzymes in cellular processes. It is particularly valuable in investigating conditions such as neurodegenerative diseases, muscle dystrophy, and ischemic injury, where calpain activity is often dysregulated. By inhibiting calpain, researchers can better understand its involvement in apoptosis, cell signaling, and cytoskeletal remodeling. Additionally, it serves as a tool in drug development to explore potential therapeutic strategies targeting calpain-related pathways. Its application extends to in vitro and in vivo studies, helping to elucidate mechanisms of disease and identify novel treatment approaches.