tert-Butyl diazoacetate is widely used in organic synthesis as a versatile reagent for the introduction of ester-functionalized carbene intermediates. It is particularly valuable in cyclopropanation reactions, where it facilitates the formation of three-membered carbon rings, a key step in the synthesis of various complex molecules. Additionally, this compound is employed in the preparation of heterocycles, which are crucial structures in pharmaceuticals and agrochemicals. Its ability to participate in C-H insertion reactions makes it a useful tool for functionalizing inert carbon-hydrogen bonds, enabling the creation of novel compounds with potential biological activity. Furthermore, tert-Butyl diazoacetate is utilized in the development of polymers and materials science, where it aids in the modification of polymer chains to achieve desired properties. Its applications also extend to the field of catalysis, where it serves as a precursor for generating metal carbenes, which are intermediates in many catalytic processes.