This compound is widely used in biomedical research for labeling proteins, peptides, and antibodies due to its near-infrared fluorescence properties. Its application is particularly valuable in in vivo imaging, as it allows for deep tissue penetration with minimal background interference. Researchers utilize it to track cellular processes, monitor drug delivery, and study tumor progression in animal models. Additionally, it is employed in flow cytometry and fluorescence microscopy to visualize and analyze specific biomolecules within complex biological systems. Its water solubility and stability make it a preferred choice for various diagnostic and experimental applications.