DiOC6(3) iodide is widely used as a fluorescent dye in biological research, particularly for studying cell membranes and organelles. It is commonly applied to label and visualize live cells, enabling researchers to observe membrane dynamics and structure under a fluorescence microscope. The dye is especially useful for tracking mitochondrial activity due to its ability to accumulate in these organelles based on membrane potential. Additionally, it is employed in flow cytometry to analyze cell viability and membrane integrity. Its lipophilic nature allows it to integrate into lipid bilayers, making it a valuable tool for studying membrane fluidity and interactions in cellular and molecular biology experiments.